GENE REGULATION AND VIRULENCE IN TB AND MAC

$337,457R01FY2003AINIH

Johns Hopkins University, Baltimore MD

Investigators

Linked publications & trials

Paper 31267892 Paper 30718426 Paper 29977244 Paper 29753789 Paper 28106876 Paper 28097645 Paper 27699232 Paper 27532494 Paper 27528266 Paper 26751217 Paper 26438491 Paper 26425693 Paper 26416658 Paper 26303802 Paper 26114158 Paper 25730264 Paper 25331694 Paper 25303934 Paper 25247851 Paper 25227469 Paper 25186281 Paper 24819223 Paper 24587015 Paper 24352433 Paper 24126586 Paper 23856770 Paper 23805786 Paper 23800952 Paper 23764389 Paper 23641020 Paper 22952680 Paper 22927424 Paper 22829866 Paper 22771606 Paper 22474037 Paper 22446041 Paper 22434196 Paper 22319585 Paper 22115541 Paper 21552395 Paper 21375756 Paper 21285434 Paper 21259445 Paper 21166899 Paper 20877639 Paper 20810423 Paper 20805989 Paper 20636328 Paper 20397943 Paper 20142044 Paper 19649276 Paper 19634702 Paper 19516256 Paper 19064634 Paper 18956986 Paper 18798983 Paper 18491971 Paper 18039768 Paper 17993538 Paper 17471433 Paper 17428787 Paper 17088352 Paper 17041849 Paper 16980499 Paper 16946269 Paper 16946268 Paper 16944099 Paper 16870781 Paper 16757153 Paper 16586367 Paper 15784600 Paper 15659067 Paper 15353557 Paper 15049808 Paper 14993625 Paper 14977982 Paper 14706097 Paper 14638810 Paper 12775759 Paper 12704132 Paper 12595455 Paper 12060776 Paper 12015690 Paper 11917811 Paper 11854245 Paper 11575983 Paper 11463228 Paper 11437339 Paper 11115701 Paper 10992456 Paper 10880571 Paper 10708372 Paper 10688144

Abstract

DESCRIPTION: (Adapted from Applicant's Abstract) This application proposes the functional characterization of mycobacterial sigma factors which the PI believes will be vital in facilitating regulated, rapid responses to the changing environments experienced by the bacilli during the course of their life cycle. The application is a continuation of an R29 award during which the PI identified sigma F (sigF) as an important regulator of loci implicated in stress and infection maintenance. Scanning of the MycD database reveals 13 putative sigma factors of which 10 are of the extracytoplasmic function group which in other systems have been shown to regulate cell wall synthesis pathways and protein secretion. The PI proposes to extend his studies by knocking out all of these sigma factors in both MTB and MAC. The knockouts will be characterized for both in vitro and in vivo phenotypes and the identity of sigma factor-dependent genes will be determined by microarray studies conducted on the knockout strains.

View original record on NIH RePORTER →