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24,576 grants matching “microbiome”
Preventing Obesity through Intervention during Infancy
$649,683Ian M Paul · Pennsylvania State Univ Hershey Med Ctr · R01 · FY2021 · DK
Gut Viral and Bacterial Associations with Celiac Disease in the TEDDY Cohort
$649,659Richard E Lloyd · Baylor College Of Medicine · R01 · FY2023 · DK
Metagenomics of the Microbiome in Oral Health and Disease
$649,618Karen E. Nelson · J. Craig Venter Institute, Inc. · R01 · FY2013 · DE
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** COCOA IS A POPULAR FOOD INGREDIENT. RESEARCH FROM OUR LABORATORY AND OTHERS HAVE SHOWN THAT DIETARY COCOA CAN MITIGATE OBESITY-RELATED INFLAMMATION AND NON-ALCOHOLIC FATTY LIVER DISEASE (NAFLD). THE EFFECTS ARE RELATED TO IMPROVED GUT HEALTH. OUR RESEARCH TEAM RECENTLY PUBLISHED TWO PAPERS SHOWING SIGNIFICANT DIFFERENCES IN THE RESPONSE OF OBESE MICE TO DIETARY COCOA BASED ON SEX. PRIOR STUDIES HAVE REPORTED THAT SEX AND SEX HORMONES CAN IMPACT DEVELOPMENT OF NAFLD AND OTHER CO-MORBIDITIES OF OBESITY IN GUTMICROBIOTA-DEPENDENT AND INDEPENDENT WAYS. BASED ON THIS, WE HYPOTHESIZE THAT DIETARY COCOA MITIGATES INFLAMMATION AND NAFLD IN OBESE MICE IN A SEX-SPECIFIC MANNER, AND THAT THE INFLUENCE OF SEX HORMONES ON THE COMPOSITION OF THE GUT MICROBIOME AND MAMMALIAN SIGNALING PATHWAYS DRIVE THESE DIFFERENCES IN BIOLOGICAL RESPONSE. WE WILLCHARACTERIZE THE SEX-SPECIFIC BENEFICIAL EFFECTS OF COCOA AGAINST OBESITY-RELATED ENTEROHEPATIC INFLAMMATION AND FATTY LIVER DISEASE. ADDITIONALLY, WE WILLCHARACTERIZE THE ROLE OF THE GUTMICROBIOME IN MEDIATING THE SEX-SPECIFIC EFFECTS OF COCOA AGAINST OBESITY-RELATED INFLAMMATION AND LIVER DISEASE.THESE STUDIES WILL PROVIDE GREATER INSIGHT INTO THE ANTI-INFLAMMATORY AND ENTEROHEPATIC PROTECTIVE ACTIVITY OF COCOA, AND THE IMPACT OF SEX AS MODERATOR. THESE DATA WILL SUPPORT THE DEVELOPMENT OF PERSONALIZED NUTRITIONAL APPROACHES AND NOVEL PRODUCTS CONTAINING COCOA TO IMPROVE HUMANHEALTH.
$649,588The Pennsylvania State University · · FY2024 · National Institute of Food and Agriculture
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** OUR TEAM RECENTLY CREATED ATOOL THAT PROVIDES ROBUST SOIL HEALTH ASSESSMENTS TO HELP LAND STEWARDS WORK TOWARDS THEIR SUSTAINABILITY GOALS AND SUPPORT HEALTHY COMMUNITIES. HOWEVER, OUR CURRENT SUITE OF SOIL HEALTH INDICATORS ARE COSTLY AND TIME-CONSUMING TO MEASURE. WHILE WE ALSO HAVENOVEL DATARESOURCES SUCH AS SOIL SPECTRA AND MICROBIOMESAT OUR FINGERTIPS, THESE DATA HAVE YET TO BE INTEGRATED INTO OUR SOIL HEALTH DATABASE, PREDICTIONS, OR EDUCATION. THEREFORE, WEPROPOSE TO IDENTIFY NOVEL DATA RESOURCES THAT ALLOW FOR MORE AFFORDABLE, RAPID, AND COMPREHENSIVE SOIL HEALTH TESTING IN DIRECT RESPONSE TO THE DATA SCIENCE FOR FOOD AND AGRICULTURE SYSTEMS CALL TO SYNTHESIZE OR ANALYZE EXISTING DATA AND RESOURCES ON SOIL HEALTH. WE WILL FIRST INTEGRATE THESE NOVEL DATA STREAMS INTO OUR CURRENT SOIL HEALTH DATABASE, AND THENTEST WHETHER THESE NEW DATA RESOURCES CAN HELP USACCURATELY PREDICT SOIL HEALTH USING MACHINE AND DEEP LEARNING MODELS.FINALLY, WE WILLTRAIN UNDERGRADUATE AND GRADUATE STUDENTS THROUGH FORMALIZED DATA SCIENCE INTERNSHIPS, COURSEWORK, AND ASSISTANTSHIPS. THROUGH THISPROJECT, WE HELP TO DEVELOP A DEEPER UNDERSTANDING OF THE RELATIONSHIP BETWEEN THE SOILFINGERPRINT (I.E., MICROBIOME AND SPECTROSCOPY) AND ITS HEALTH STATUS, AN IMPROVED TOOL TO EFFECTIVELY MEASURE SOIL HEALTH, AND THE DEVELOPMENT OF A SKILLED WORKFORCE. OURCOMBINED EXPERTISE CREATES A UNIQUE OPPORTUNITY TO LEVERAGE EXISTING RESOURCES AND MAKE ADVANCEMENTS IN RESEARCH AND EDUCATION THAT WILL BENEFIT STUDENTS, LAND STEWARDS, THE NEXT GENERATION OF RESEARCHERS, AND THE COMMUNITY AT LARGE.
$649,570University Of Hawaii · · FY2023 · National Institute of Food and Agriculture
Microbiota, Metabolites, and Colon Neoplasia
$649,547Daniel W Rosenberg · University Of Connecticut Sch Of Med/Dnt · R01 · FY2022 · CA
Age and Diet: Major interacting factors that drive sporadic intestinal cancer
$649,333Leonard H. Augenlicht · Albert Einstein College Of Medicine, Inc · R01 · FY2015 · CA
1/2 Alcohol Associated Comorbidities and Microbiome Evaluation in HIV (ACME HIV)
$649,329Matthew S Freiberg · Vanderbilt University Medical Center · U01 · FY2019 · AA
Frailty, HIV Infection, Injection Drug Use and the Inflammatory-Microbiome
$649,179Damani Piggott · Johns Hopkins University · R01 · FY2018 · AG
Elucidating the role of gut microbiota in colitis-associated colorectal cancer
$649,138Ru Chen · Baylor College Of Medicine · R01 · FY2023 · CA
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** RATIONALEA DAUNTING CHALLENGE FACED BY FARMING COMMUNITIES AND ANIMAL SCIENTISTS IS TO DEFINE ANIMAL WELFARE STANDARDS OWING TO THE ETHICAL, ECONOMIC, AND SCIENTIFIC DEBATES AROUND CARE AND MANAGEMENT OF AGRICULTURAL ANIMALS. EFFORTS HAVE FOCUSED ON REDUCING STRESS, PAIN, ANXIETY, AND DEVIATION FROM NATURE IN INTENSIVE FARMING SYSTEMS, ALL OF WHICH HAVE BECOME SOCIETAL CONCERNS. A RISING TREND IN ANIMAL WELFARE IS ALSO TO PROMOTE A GOOD LIFE WHICH INCLUDES POSITIVE AFFECTIVE STATES. HOWEVER, QUESTIONS REMAIN ON THE MECHANISTIC BASIS INFLUENCING DIFFERENT TYPES OF BEHAVIOR. RESEARCH IN HUMANS HAS DEMONSTRATED THAT THE EARLY LIFE MICROBIOME IS ESSENTIAL FOR PROPER DEVELOPMENT OF THE ENTERIC AND CENTRAL NERVOUS SYSTEMS, THE IMMUNE SYSTEM, AND THE MICROBIOME-GUT-BRAIN AXIS (MGBA). FURTHER, EARLY LIFE MICROBIAL DYSBIOSIS MAY LEAD TO AN INCREASED INCIDENCE OF DISEASED STATES, INCLUDING BEHAVIORAL ISSUES, LATER IN LIFE. SUCH STUDIES HAVE NOT BEEN REPORTED IN AGRICULTURAL ANIMALS. THE PREMISEFOR THIS GRANT IS THAT STRONG BI-DIRECTIONAL CONNECTIONS EXIST BETWEEN MICROBIOME-GUT-BRAIN AXIS (MGBA) THAT THEN EFFECTS ANIMAL BEHAVIOR IN DAIRY CATTLE.HYPOTHESISTHE OVERALL GOAL OF THIS RESEARCH IS TO DETERMINE IF FAECALIBACTERIM PRAUSNITZII WHEN USED IN CALVES CAN ALTER THE IMMUNE RESPONSE, REDUCE STRESS-INDUCED RESPONSES AND HAVE LASTING EFFECTS ON POSITIVE BEHAVIOR IN DAIRY CATTLE. WE HYPOTHESIZE THAT PERTURBING GUT MICROBIOTA (DYSBIOSIS) EARLY IN DAIRY CALVES' LIVES MAY INDUCE SYSTEMIC CHANGES LEADING TO ALTERATIONS IN BEHAVIOR AND THAT SUPPLEMENTATION WITH FAECALIBACTERIUM PRAUSNITZII, A BUTYRATE PRODUCING BACTERIUM MAY PREVENT MICROBIAL DYSBIOSIS AND REDUCE STRESS-INDUCED BEHAVIORS THUS IMPROVING HEALTH, WELL-BEING, AND WELFARE.SPECIFIC OBJECTIVESOBJECTIVE 1: DETERMINE THE EXTENT TO WHICH EARLY-LIFE MICROBIAL DYSBIOSIS ALTERS PHYSIOLOGICAL RESPONSES AND BEHAVIOR (MICROBIOME-GUT-BRAIN AXIS) AND IF SUCH ALTERATIONS ARE REVERTED BY DOSING CALVES WITH F. PRAUSNITZII IN DAIRY CALVES AND SUSTAINED FOR LONGER PERIODS. OBJECTIVE 2: DETERMINE THE EFFECTS OF DOSING F. PRAUSNITZII ON REVERSING STRESS-INDUCED BEHAVIORS SUCH AS DEHORNING AND TRANSPORTATION IN DAIRY CALVES.THESE TWO OBJECTIVES WILL BE ACCOMPLISHED BY PERFORMING TWO ANIMAL EXPERIMENTS. THE STUDY WILL USE DAIRY CALVES. THE FIRST PHASE TO DETERMINE IMMUNE AND BEHAVIORAL RESPONSES WILL USE NEWBORN CALVES AND THEY WILL BE DIVIDED INTO 3 GROUPS: ORAL ANTIBIOTICS, ORAL ANTIBIOTIC PLUS PROBIOTIC AND CONTROL GROUP. THE CALVES WILL STAY IN THE STUDY FOR 24 MONTHS. THE 2ND PHASE TO DETERMINE PROBIOTIC EFFECTS ON STRESS INDUCED RESPONSES WILL USE 5-6 WEEK OLD CALVES. THE CALVES WILL BE IN TWO GROUPS: ORAL PROBIOTIC AND CONTROL. THESE CALVES WILL BE ENROLLED FOR 12 WEEKS.EXPECTED RESULTSWE ANTICIPATE THAT THIS PROJECT WILL AUGMENT OUR CURRENT RESEARCH ACTIVITIES AND ENHANCE OUR KNOWLEDGE OF THE ROLE OF THE GUT MICROBIOME IN IMPROVING HEALTH, WELL-BEING, WELFARE, AND PRODUCTION PERFORMANCE IN AGRICULTURAL ANIMALS.,USING STRESSORS AND POSITIVE WELFARE TESTS COMBINED WITH MICROBIOME, METABOLOMIC, AND SYSTEMIC RESPONSES, WE WILL DETERMINE THE LINK BETWEEN THE MGBA AND BEHAVIOR AND ALSO DETERMINE THE EFFICACY OF F. PRAUSNITZII AS A PSYCHOBIOTIC TO IMPROVE HEALTH AND BEHAVIOR IN DAIRY CATTLE.ANTICIPATED IMPACTWITH A STRONG, INTERDISCIPLINARY TEAM OF VETERINARIANS, WE ANTICIPATE THIS PROJECT WILL BE A SCIENTIFIC BREAKTHROUGH IN DEVELOPING NOVEL EARLY LIFE MICROBIAL INTERVENTIONS TO IMPROVE HEALTH, WELFARE, AND WELL-BEING OF DAIRY CATTLE VIA CHOREOGRAPHING DEVELOPMENT OF THE MGBA.
$649,000Trustees Of The University Of Pennsylvania, The · · FY2023 · National Institute of Food and Agriculture
HIV-Associated Lung Disease: The Role of Nasal Mucosal Immunity and Microbiome
$648,939Michael Bradley Drummond · Univ Of North Carolina Chapel Hill · R01 · FY2024 · HL
Esophageal cancer genetic project
$648,853Alisa Goldstein · Division Of Cancer Epidemiology And Genetics · ZIA · FY2024 · CA
Next Generation of HPV and Cervical Cancer Research in HIV+ Women
$648,844Howard D Strickler · Albert Einstein College Of Medicine · R01 · FY2020 · CA
Dietary fiber and soy protein-based microbiome metabolites for IBD prevention
$648,836Eric C Martens · University Of Michigan At Ann Arbor · R01 · FY2024 · DK
Folic Acid Supplementation and Colitis-associated Colon Carcinogenesis
$648,814Margie L. Clapper · Research Inst Of Fox Chase Can Ctr · R01 · FY2025 · CA
Impact of benzalkonium chloride on gut microbiome and gut-liver interactions
$648,691Libin Xu · University Of Washington · R01 · FY2024 · ES
Impact of benzalkonium chloride on gut microbiome and gut-liver interactions
$648,691Libin Xu · University Of Washington · R01 · FY2025 · ES
Targeting tumoral Lactobacillus iners to improve outcomes in cervical cancers
$648,677Lauren Elizabeth Colbert · University Of Tx Md Anderson Can Ctr · R37 · FY2025 · CA
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** THE HIGH-MORTALITY PATHOGEN LISTERIA MONOCYTOGENES IS ENDEMIC AND CAN EASILY BE INTRODUCED INTO MEAT PROCESSING FACILITIES, BUT THE CONDITIONS THAT ALLOW IT TO PERSIST IN THESE LOCATIONS ARE NOT FULLY KNOWN. LISTERIA MONOCYTOGENES IS A BACTERIAL PATHOGEN ASSOCIATED WITH READY-TO-EAT MEATS, SOFT CHEESES, ICE CREAM, AND MANY OTHER FOOD PRODUCTS. IT IS OF PARTICULAR CONCERN DUE TO A 30% MORTALITY RATE AND ELEVATED RISKS OF PREGNANCY LOSS AND LONG-TERM DISABILITY. DESPITE MONUMENTAL EFFORTS BY THE FOOD INDUSTRY TO PREVENT CONTAMINATION, THERE ARE REGULAR OUTBREAKS OF LISTERIOSIS, ESPECIALLY AMONG THE YOUNG, OLD, AND IMMUNOCOMPROMISED. IN JUST THE LAST FIVE YEARS IT HAS BEEN THE CAUSATIVE AGENT FOR AT LEAST 15 OUTBREAKS, RESULTING IN 194 ILLNESSES, 176 HOSPITALIZATIONS, AND 18 DEATHS. THESE OUTBREAKS ARE NOT ONLY DEVASTATING IN TERMS OF PUBLIC HEALTH, BUT THEY ALSO LEAD TO LARGE FOOD PRODUCT RECALLS, WHICH HAVE A HIGH ECONOMIC COST AND CONTINUETO EXASPERATE THE GLOBAL FOOD WASTE PROBLEM. THE RISK THIS PATHOGEN POSES TO OUR MOST VULNERABLE POPULATIONS MAKES IT WORTH THE CONTINUED SCIENTIFIC PURSUIT OF NEW METHODS TO IDENTIFY AND PREVENT POTENTIAL OUTBREAKS BEFORE THEY BEGIN.UNDERSTANDING WHY LISTERIA PERSISTS IN SOME MEAT PROCESSING FACILITIES IS CRITICAL TO ADDRESS THIS PUBLIC HEALTH ISSUE. THESE FACILITIES UNDERGO RIGOROUS DAILY CLEANING AND SANITATION PROTOCOLS, AND THIS HOSTILE ENVIRONMENT HAS SELECTED FOR ORGANISMS THAT HAVE SURVIVAL ADAPTATIONS; ONE SUCH ADAPTATION IS THE ABILITY TO FORM BIOFILMS, A STRUCTURED NETWORK OF PROTEIN AND SUGARS THAT PROTECTS THE ORGANISMS LIVING WITHIN IT. THESE BIOFILMS CAN PROVIDE A PERFECT ENVIRONMENTAL NICHE FOR LISTERIA PERSISTENCE. HOWEVER, WE STILL KNOW VERY LITTLE ABOUT THE ROLE OF SPECIFIC ORGANISMS IN A BIOFILM MICROBIOME AND THEIR POTENTIAL IMPACTS ON FOOD PRODUCTS. THEREFORE, IN THIS PROJECT, WE WILL DETERMINE WHETHER THERE ARE MEAT PROCESSING FACILITY-ASSOCIATED BIOFILM MICROBIAL COMMUNITIES THAT SUPPORT OR INHIBIT THE GROWTH AND SURVIVAL OF LISTERIA MONOCYTOGENES.TO ADDRESS THIS CRITICAL RESEARCH QUESTION, WE WILL EMPLOY DNA SEQUENCING AND 'OMICS TECHNOLOGIES TO FULLY REVEAL THE POPULATIONS AND ACTIVITIES OF THE MICROBES INVOLVED IN FACILITY BIOFILMS. WE WILL WORK WITH THE MEAT PROCESSING COMMUNITY TO COLLECT BIOFILM SAMPLES FROM SMALL MEAT FACILITIES LOCATED IN ALABAMA AND THE SOUTHEASTERN UNITED STATES. WITHIN EACH FACILITY, WE WILL PLACE STAINLESS STEEL COUPONS IN SELECT DRAINS THROUGHOUT THE PROCESSING SPACES AND ALLOW BIOFILMS TO GROW OVER A PERIOD OF THREE MONTHS, WHICH WILL ALLOW US TO FORM A BIO-MAP OF BIOFILM MICROBES REPRESENTATIVE OF ALL SMALL PROCESSORS. ONCE WE HAVE COLLECTED THE SAMPLES, WE WILL DIVIDE THE COUPON INTO THREE PORTIONS. THE FIRST WILL UNDERGO DNA SEQUENCING, WHICH WILL ALLOW US TO IDENTIFY THE SPECIFIC MICROORGANISMS PRESENT IN THE SAMPLE, THE RELATIVE ABUNDANCE OF EACH ORGANISM IN THE COMMUNITY, AND THEIR OVERALL MICROBIAL DIVERSITY. THE SECOND PORTION WILL UNDERGO RNA SE,QUENCING, WHICH WILL ALLOW US TO DETERMINE THE BIOLOGICAL FUNCTIONS THESE ORGANISMS ARE GENERATING (E.G., BIOFILM FORMATION, COMPETITION). THE FINAL PORTION WILL BE CULTURED WITH LISTERIA MONOCYTOGENES, AND THEN ALSO UNDERGO RNA SEQUENCING, SO WE CAN DETERMINE WHAT MICROBIAL ACTIVITIES CHANGE IN RESPONSE TO THE ADDITION OF THE PATHOGEN. WE HYPOTHESIZE THAT THE ADDITION OF LISTERIA TO THESE COMMUNITIES WILL DRIVE A RE-ORGANIZATION OF THE COMMUNITY, WHICH WILL BE EVIDENT BY THESE FUNCTIONAL CHANGES. DIFFERENT COMMUNITIES WILL LIKELY HAVE DIFFERENT RESPONSES, AND WITH THIS INFORMATION WE CAN IDENTIFY BIOFILM COMMUNITIES MORE LIKELY TO RESPOND WITH COMPETITIVE EXCLUSION TO LISTERIA, THEREFORE PREVENTING ITS COLONIZATION.THE GOAL OF THIS RESEARCH PROJECT IS TO BETTER UNDERSTAND THE DYNAMICS OF MICROBES WITHIN MEAT PROCESSING FACILITY BIOFILMS. OUR METHODS WILL ALLOW US TO DIRECTLY ADDRESS THE DIVERSITY OF MICROBIAL COMMUNITIES IN THESE ENVIRONMENTS ACROSS THE REGION AND TO GAIN INSIGHT INTO WHY THEY FORM AND WHAT ROLES EACH MEMBER PERFORMS. WITH THE INTRODUCTION OF AN IMPORTANT PATHOGEN, LISTERIA MONOCYTOGENES, WE WILL ALSO LEARN HOW THESE COMMUNITIES RESPOND TO THE INTRODUCTION OF NEW ORGANISMS, AND WHETHER THIS INCREASES THE AMOUNT OF COMPETITION FOR NUTRIENTS WITHIN THE LIMITED SPACE. ULTIMATELY, WE BELIEVE THIS WILL LEAD TO THE CREATION OF AN EARLY-WARNING SYSTEM FOR DETECTING THE GROWTH POTENTIAL OF LISTERIA IN A HIGH-RISK SPACE SUCH AS MEAT PROCESSING FACILITIES BEFORE IT IS ACTUALLY COLONIZED BY THE PATHOGEN. THROUGH THIS, WE WILL AIM TO DECREASE THE RISK OF PATHOGEN CONTAMINATION FOR THE SMALL PROCESSOR, DECREASE THE ECONOMIC BURDEN ASSOCIATED WITH FOOD RECALLS AND ILLNESS, AND IMPROVE THE SAFETY OF FOOD PRODUCTS LOCALLY, REGIONALLY, AND, ULTIMATELY, NATIONWIDE.
$648,627Auburn University · · FY2023 · National Institute of Food and Agriculture
RAISE-SitS: Designing models to forecast how biogeochemical fluctuations in soil systems govern soil development, terrestrial water storage and ecosystem nutrient fluxes
$648,574Pamela L Sullivan · Oregon State University · · FY2019 · GEO
Blocking CMV transmission through the human milk metabolome and microbiome
$648,545Tatiana T. Marquez-Lago · University Of Alabama At Birmingham · R01 · FY2021 · HD
Microbiome Affects Risk of Growth in HIV-exposed but Unifected Infants-Nigeria
$648,430Manhattan E Charurat · University Of Maryland Baltimore · R01 · FY2014 · DE
Lubricant Use and the Vaginal Microbiome
$648,397Rebecca M. Brotman · University Of Maryland Baltimore · R01 · FY2020 · AI
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** SALMONELLA IS A SIGNIFICANT PUBLIC HEALTH THREAT AND ECONOMIC CONCERN AS FOODBORNE ILLNESS CONTINUES TO BE AN OVERWHELMING CHALLENGE IN THE U.S. WHILE NUMEROUS INTERVENTION STRATEGIES ARE USED IN POULTRY PROCESSING TO REDUCESALMONELLAPOPULATIONS WITH SOME SUCCESS, P LIVE BIRD PRODUCTION REMAINS A CRITICAL STEPFORSALMONELLACONTROL. USING8 TO12 HOUR FEED WITHDRAWAL (FW) BEFORE THEBROILERS ENTERTHE PROCESSING PLANTTO REDUCE FECAL CONTAMINATION APPEARS TO BE A MAJOR FACTOR TO INCREASES IN SALMONELLA. OUR RESEARCH PROPOSAL REPRESENTS AN NEWWAY FOR SALMONELLA CONTROL WITH ATARGETED BIOENGINEERING DESIGNOF A MULTIPLE PHAGE POPULATION WITH AN OPTIMIZEDSALMONELLA-KILLINGIMPACT THAT CAN BE APPLIED DURING FW TO REDUCE MOST IF NOT ALLSALMONELLA SEROVARS AND STRAINS.OURGENERAL HYPOTHESISIS THAT BIOENGINEERING PHAGES WITH DIFFERENT SALMONELLAATTACHMENT SITES ALLOW FOR THEIR MAXIMUM AND CONTINUOUS EXPOSURE TO TARGETSALMONELLA PREVENTINGHORIZONTALSALMONELLATRANSMISSIONAMONG BIRDS DURING FW REDUCING SALMONELLA PREVALENCE AT THE PROCESSING PLANT.OUR SPECIFIC OBJECTIVES:OBJECTIVE 1- BIOENGINEER BACTERIOPHAGES FOR OPTIMAL EFFICACY AGAINSTSALMONELLASEROVARS DURING FW.OBJECTIVE 2 -SCREEN THE RESULTING BIOENGINEERED PHAGE CANDIDATES FROMOBJECTIVE 1AGAINST MULTIPLESALMONELLASEROVARS USING IN VITRO CROP AND CECAL INCUBATIONS.OBJECTIVE 3- DETERMINE THE EFFICACY OFSALMONELLAPHAGE COCKTAIL IDENTIFIED IN OBJECTIVE 2 SUPPLEMENTED IN THE DRINKING WATER OF COMMERCIAL BROILERS ON REDUCINGSALMONELLATRANSMISSION DURING FW AND SALMONELLA LOAD AT THE PROCESSING PLANT.THE RESULTS FROM THIS RESEARCH OCCUR AT A MOST OPPORTUNE TIME FOR THE MEAT AND POULTRY INDUSTRY AS THE INDUSTRY IS NOW CONSIDERING BACTERIOPHAGES AS A POTENTIAL APPROACH FOR LIMITING PATHOGENS. MOREOVER, THE INDUSTRY IS DESPERATE FOR VIABLE OPTIONS. OUR COLLABORATIVE APPROACH CONCEPTUALLY REPRESENTS A HIGHLY TARGETED STRATEGY BY BIOENGINEERING MORE EFFICACIOUS PHAGE FOR THE POULTRY GIT AND TAILORED FOR APPLICATION DURING FW BEFORE POULTRY PROCESSING. IN ADDITION, OUR BIOENGINEERED PHAGE APPROACH REPRESENTS A BROAD-SPECTRUM INTERVENTION TO MOST SEROVARS BOTH CURRENTLY PREVALENT ONES AS WELL AS EMERGING SEROVARS. POULTRY IS ONLY THE BEGINNING. SALMONELLA IS ALSO PREVALENT IN SWINE, CATTLE, AND NONMEAT FOODS SUCH AS VEGETABLES. WITH TRADITIONAL INTERVENTIONS, SALMONELLA CAN BE ESPECIALLY DIFFICULT TO TACKLE IN SWINE AND CATTLE ONCE THEY INVADE DEEP TISSUES WHERE PHAGE APPLICATION COULD BE PRACTICAL. FINALLY, FROM MORE OF A LONG-TERM STANDPOINT, THIS APPROACH OPENS THE FLOODGATES TO TACKLE OTHER PATHOGENS WHICH ARE ALSO HIGHLY PROBLEMATIC FOR THE POULTRY INDUSTRY, SUCH AS CAMPYLOBACTER, WHICH RESIDES IN THE CHICKEN GUT AS PART OF THE MICROBIOME AND IS NEARLY IMPOSSIBLE TO TARGET WITH ANTIMICROBIALS.
$648,244University Of Wisconsin System · · FY2024 · National Institute of Food and Agriculture