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17,828 grants matching “crispr”
Cancer Center Support (Core) Grant
$3,572,644Tyler E Jacks · Massachusetts Institute Of Technology · P30 · FY2018 · CA
Cancer Center Support (Core) Grant
$3,572,644Tyler E Jacks · Massachusetts Institute Of Technology · P30 · FY2016 · CA
Cancer Center Support (Core) Grant
$3,572,644Tyler E Jacks · Massachusetts Institute Of Technology · P30 · FY2019 · CA
Cancer Center Support (Core) Grant
$3,572,644Tyler E Jacks · Massachusetts Institute Of Technology · P30 · FY2015 · CA
Genetic epidemiology of complex diseases
$3,555,403Charles Rotimi · National Human Genome Research Institute · ZIA · FY2021 · HG
Comparative Analysis Of Completely Sequenced Genomes
$3,513,696Eugene V Koonin · National Library Of Medicine · ZIA · FY2023 · LM
Cross Scale Interrogation of NPD Genes (SING) in mice
$3,498,931Yongsoo Kim · Pennsylvania State Univ Hershey Med Ctr · RM1 · FY2025 · MH
Molecular Mechanisms and Genetic Basis of Human Ovarian Aging
$3,445,585Yousin Suh · Columbia University Health Sciences · RF1 · FY2025 · AG
Consolidated Biomedical Core Facilities Supporting a Center on Emerging and Zoonotic Infectious Disease Research
$3,430,993Bonnie Rush · Kansas State University · C06 · FY2021 · OD
Genetic epidemiology of complex diseases
$3,386,972Charles Rotimi · National Human Genome Research Institute · ZIA · FY2019 · HG
TREAT AD Assay Core
$3,382,783Allan I Levey · Emory University · U54 · FY2024 · AG
WE PROPOSE TO GENERATE NON-TRANSGENIC HLB RESISTANT CITRUS VARIETIES VIA GENOME EDITING OF CITRUS SUSCEPTIBILITY (S) GENES TO HLB USING CRISPR/CAS9 TECHNOLOGY. OUR CENTRAL HYPOTHESIS IS THAT CANDIDATUS LIBERIBACTER ASIATICUS (LAS), WHICH UTILIZES A SET OF BACTERIAL SECRETED EFFECTOR PROTEINS TO MANIPULATE KEY DISEASE S GENE(S), CAN BE ELIMINATED BY MODIFICATION OF KEY S GENE(S). WE PROPOSE TO CHARACTERIZE THE INTERACTION BETWEEN LAS EFFECTORS AND KEY S GENES OR THEIR PRODUCTS. MULTIPLE CANDIDATE S GENES BASED ON INTERACTIONS WITH KEY EFFECTORS HAVE BEEN IDENTIFIED IN OUR PRELIMINARY STUDY. WE WILL GENERATE MUTATIONS IN THE CANDIDATE S GENES USING CRISPR-CAS9 AND SCREEN THE MUTANTS FOR RESISTANCE TO HLB. THE CONFIRMED S GENE(S) WILL BE MODIFIED IN ADDITIONAL SELECTED CITRUS SCION AND ROOTSTOCK VARIETIES. IN PARALLEL, WE WILL CONSTRUCT MUTATIONS IN THE KEY S GENES TO PINPOINT THE CRITICAL RESIDUES FOR INTERACTIONS WITH LAS EFFECTORS, WHICH WILL ASSIST IN DIRECTING PLANT MODIFICATIONS AND VALIDATION OF IDENTIFIED RESISTANT LINES. THIS PROPOSAL ADDRESSES ONE OF THE SIX AREAS OF HIGHEST PRIORITY DETERMINED BY THE CITRUS DISEASE SUB-COMMITTEE (CDS): DEVELOPMENT OF TOLERANCE OR RESISTANCE TO HLB IN CULTIVARS COMMERCIALLY IMPORTANT IN ALL CITRUS PRODUCTION REGIONS. THE STRATEGY TO BE EMPLOYED AND THE OBJECTIVES ARE DEVELOPED BASED ON INPUT FROM CITRUS INDUSTRY. CITRUS GROWER SPONSORED PROJECTS ESTABLISHED THE FOUNDATION OF THIS APPLICATION, E.G., ADOPTION OF CRISPR TECHNOLOGY ON CITRUS. THE NON-TRANSGENIC PLANTS GENERATED HERE MAY NOT BE REGULATED BY USDA AND COULD BE COMMERCIALIZED IMMEDIATELY, IF PROVEN HLB RESISTANT AND WITH ACCEPTABLE HORTICULTURAL TRAITS.
$3,378,628University Of Florida · · FY2018 · National Institute of Food and Agriculture
Defining the impact of type 1 cytokine signaling on STAT-mediated epithelial reprogramming and barrier dysfunction in eosinophilic esophagitis
$3,375,359Melanie A Ruffner · Children'S Hosp Of Philadelphia · R01 · FY2025 · AI
Structure-Function Analysis of Mxra8 Interaction with Alphaviruses
$3,371,896Michael S. Diamond · Washington University · R01 · FY2025 · AI
Functional and Mechanistic Characterization of Lupus Susceptibility
$3,364,935Swapan K Nath · Oklahoma Medical Research Foundation · R01 · FY2025 · AI
Understanding the complexity of gene dosage imbalance in Down syndrome
$3,352,210Stella T Chou · Children'S Hosp Of Philadelphia · R01 · FY2019 · HL
Comparative Analysis Of Completely Sequenced Genomes
$3,340,583Eugene V Koonin · National Library Of Medicine · ZIA · FY2012 · LM
Transgenic Resources for Neuroscience Research
$3,319,825James Pickel · National Institute Of Mental Health · ZIC · FY2017 · MH
AAV-mediated editing to treat human autosomal dominant hearing loss DFNA41 and DFNA2
$3,314,560Zheng-Yi Chen · Massachusetts Eye And Ear Infirmary · U01 · FY2024 · TR
Comparative Analysis Of Completely Sequenced Genomes
$3,296,590Eugene V Koonin · National Library Of Medicine · ZIA · FY2022 · LM
E-RISE RII: Cracking the developmental blueprint of life: Omics, computational science, and artificial intelligence
$3,296,580Riccardo Papa · University Of Puerto Rico-Rio Piedras · · FY2024 · O/D
Mechanisms of inherited neurodegenerative diseases
$3,289,479Michael Ward · National Institute Of Neurological Disorders And Stroke · ZIA · FY2021 · NS
B cell development
$3,276,931Rafael C Casellas · National Institute Of Arthritis And Musculoskeletal And Skin Diseases · ZIA · FY2017 · AR
Mechanisms, modeling and therapies of retinal and macular neurodegeneration
$3,271,791Anand Swaroop · National Eye Institute · ZIA · FY2023 · EY
Human immune signatures of Dengue virus and Mycobacterium Tuberculosis exposure in infection, disease and vaccination
$3,238,555Alessandro Sette · La Jolla Institute For Immunology · U19 · FY2017 · AI